GERI-BP002-A, novel inhibitor of acyl-CoA: cholesterol acyltransferase produced by Aspergillus fumigatus F93

J Antibiot (Tokyo). 1996 Jan;49(1):31-6. doi: 10.7164/antibiotics.49.31.

Abstract

A new inhibitor of acyl-CoA:cholesterol acyltransferase (ACAT), designated GERI-BP002-A, was isolated from the culture broth of Aspergillus fumigatus F93 by acetone extraction, EtOAc extraction, SiO2 column chromatography and reverse phase HPLC. Spectroscopic analyses of the compound identified bis (2-hydroxy-3-tert-butyl-5-methylphenyl) methane as the structure and its molecular weight and formula to be 340 and C23H32O2, respectively. GERI-BP002-A inhibited ACAT activity by 50% at the concentration of 50 microM in an enzyme assay system using rat liver microsomes.

MeSH terms

  • Aspergillus fumigatus / classification
  • Aspergillus fumigatus / metabolism*
  • Butylated Hydroxytoluene / analogs & derivatives*
  • Butylated Hydroxytoluene / isolation & purification
  • Enzyme Inhibitors / chemistry
  • Enzyme Inhibitors / isolation & purification*
  • Enzyme Inhibitors / pharmacology
  • Fermentation
  • Humans
  • Sterol O-Acyltransferase / antagonists & inhibitors*
  • Tumor Cells, Cultured

Substances

  • Enzyme Inhibitors
  • Butylated Hydroxytoluene
  • Sterol O-Acyltransferase
  • 2,2'-methylenebis(4-methyl-6-tert-butylphenol)