Cell-mediated immunity to pseudorabies virus: cytolytic effector cells with characteristics of lymphokine-activated killer cells lyse virus-infected and glycoprotein gB- and gC-transfected L14 cells

J Gen Virol. 1996 May;77 ( Pt 5):987-90. doi: 10.1099/0022-1317-77-5-987.

Abstract

We examined cytolytic cells that lyse pseudorabies virus (PRV)-infected cells in pigs. In vitro stimulation of peripheral blood mononuclear cells from PRV-immune pigs with live PRV generated cells that lysed PRV-infected immortalized B cells. Several lines of evidence indicated a major contribution of non-major histocompatibility complex (MHC)-restricted cytolytic cells, which displayed characteristics of natural killer (NK) or lymphokine-activated killer cells: cytolysis was non-MHC-restricted, depended on CD2+CD4-CD8bright- (or CD2+CD4-CD8dull+) cells, was strongly augmented by in vitro antigenic stimulation and was not limited to virus-infected cells, i.e. the NK cell-susceptible target cell line K562 was also lysed. Cytolytic cells were also generated by in vitro antigenic stimulation with UV-inactivated PRV. Target cells transfected with and stably expressing PRV gB or gC were lysed to the same degree as PRV-infected target cells.

MeSH terms

  • Animals
  • Cell Line
  • Cytotoxicity, Immunologic*
  • Herpesvirus 1, Suid / immunology*
  • Immunity, Cellular
  • Killer Cells, Lymphokine-Activated / immunology*
  • Swine
  • T-Lymphocytes, Cytotoxic / immunology
  • Transfection
  • Viral Envelope Proteins / genetics
  • Viral Envelope Proteins / physiology*

Substances

  • Viral Envelope Proteins
  • pseudorabies virus glycoproteins