Determination of epitestosterone and testosterone in urine by high-performance liquid chromatography

J Chromatogr B Biomed Appl. 1995 Nov 17;673(2):159-64. doi: 10.1016/0378-4347(95)00260-x.


This paper describes an alternative HPLC method for the determination of testosterone and epitestosterone, which is simple, rapid, selective, sensitive and reproducible. Samples were prepared using a previous enzymatic hydrolysis with liquid-liquid extraction. The determination was carried out on a Hypersil BDS-C18 reversed-phase column with gradient elution and UV absorbance detection (240 nm). The limits of quantification (signal-to-noise ratio = 6) were 20 ng/ml for testosterone and 30 ng/ml for epitestosterone.

MeSH terms

  • Chromatography, High Pressure Liquid / methods*
  • Chromatography, High Pressure Liquid / statistics & numerical data
  • Epitestosterone / urine*
  • Humans
  • Hydrogen-Ion Concentration
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Testosterone / urine*


  • Testosterone
  • Epitestosterone