Deletion of the Ig kappa light chain intronic enhancer/matrix attachment region impairs but does not abolish V kappa J kappa rearrangement

Immunity. 1996 Apr;4(4):377-85. doi: 10.1016/s1074-7613(00)80251-4.

Abstract

Roles of the kappa intronic enhancer (iE kappa) and its associated matrix attachment region (MAR) during B cell development were examined using mutant embryonic stem (ES) cell lines in which the entire region on both chromosomes was replaced with either a recombined LoxP site (E kappa ND) or the PGK-neomycin resistance (PGK-neo(r)) gene (E kappa NI). B cells derived from E kappa ND ES cells had greatly impaired V kappa J kappa rearrangement, normal levels of kappa expression, and kappa:lambda ratios of 1:1 instead of the usual 10:1. Furthermore, lambda-producing hybridomas derived from E kappa ND cells displayed little kappa rearrangement. Thus, the MAR and iE kappa are quantitatively significant for kappa rearrangement but not necessary. In addition, little V kappa J kappa rearrangement could be detected in B cells derived from E kappa NI ES cells, demonstrating that an inserted PGK-neo(r) gene dominantly suppresses V kappa J kappa rearrangement.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • B-Lymphocytes / cytology
  • B-Lymphocytes / immunology
  • Base Sequence
  • Binding Sites / genetics
  • Cell Differentiation
  • Cell Line
  • Chimera / genetics
  • Chimera / immunology
  • DNA Primers / genetics
  • Enhancer Elements, Genetic
  • Gene Rearrangement, B-Lymphocyte, Light Chain*
  • Hybridomas / immunology
  • Immunoglobulin kappa-Chains / genetics*
  • Introns
  • Mice
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Sequence Deletion

Substances

  • DNA Primers
  • Immunoglobulin kappa-Chains