Resistance to endotoxin shock and reduced dissemination of gram-negative bacteria in CD14-deficient mice

Immunity. 1996 Apr;4(4):407-14. doi: 10.1016/s1074-7613(00)80254-x.


Endotoxin shock is the result of activation of the immune system by endotoxin/LPS, a component of Gram-negative bacteria. CD14, a GPI-anchored glycoprotein expressed strongly by monocyte/macrophages, is one of several receptors for endotoxin/LPS. The role of CD14 in bacterial-induced and LPS-induced shock was tested in CD14-deficient mice produced by gene targeting in embryonic stem cells. CD14-deficient mice were found to be highly resistant to shock induced by either live Gram-negative bacteria or LPS; however, at very high concentrations of LPS or bacteria, responses through non-CD14 receptors could be detected. Surprisingly, CD14-deficient mice also showed dramatically reduced levels of bacteremia, suggesting an unexpected role for CD14 in the dissemination of Gram-negative bacteria.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cytokines / biosynthesis
  • Escherichia coli Infections / genetics
  • Escherichia coli Infections / immunology
  • Escherichia coli Infections / prevention & control*
  • Gene Targeting
  • Lipopolysaccharide Receptors / genetics*
  • Lipopolysaccharide Receptors / metabolism*
  • Lipopolysaccharides / toxicity
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Monocytes / drug effects
  • Monocytes / immunology
  • Shock, Septic / genetics
  • Shock, Septic / immunology
  • Shock, Septic / prevention & control*


  • Cytokines
  • Lipopolysaccharide Receptors
  • Lipopolysaccharides