Toward Cell-Targeting Gene Therapy Vectors: Selection of Cell-Binding Peptides From Random Peptide-Presenting Phage Libraries

Nat Med. 1996 Mar;2(3):299-305. doi: 10.1038/nm0396-299.

Abstract

Ideal gene therapy vectors would be delivered intravenously to transfect only specific cells. Existing vectors only transfect cells in vivo in a manner determined by blood flow and the site of introduction. As a general and systematic approach for generating cell-targeting ligands for gene therapy vectors, we have used peptide-presenting phage libraries to select peptides that bind and enter several different cell types. Because of their small size, cell-binding peptides such as these could be incorporated into biological or physical gene therapy vectors. In addition, peptide-presenting phage themselves may also be candidates for gene therapy vectors.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Bacteriophages / genetics
  • Binding, Competitive
  • Cell Line
  • Fibroblasts / metabolism
  • Gene Library*
  • Gene Targeting / methods*
  • Genetic Therapy / methods*
  • Genetic Vectors*
  • Immunohistochemistry
  • Mice
  • Molecular Sequence Data
  • Molecular Structure
  • Mutation
  • Peptides / chemistry
  • Peptides / genetics*
  • Peptides / metabolism*
  • Protein Binding
  • Protein Conformation

Substances

  • Peptides