LET-23 receptor localization by the cell junction protein LIN-7 during C. elegans vulval induction

Cell. 1996 Apr 19;85(2):195-204. doi: 10.1016/s0092-8674(00)81096-x.


In C. elegans, the anchor cell signal induces Pn.p cells to form the vulva by activating a conserved receptor tyrosine kinase pathway. lin-2 and lin-7 mutants exhibit a vulvaless phenotype similar to the phenotype observed when this signaling pathway is defective. We have found that LIN-7 is a cell junction-associated protein that binds to the LET-23 receptor tyrosine kinase. LET-23 is also localized to the cell junctions, and both LIN-2 and LIN-7 are required for this localization. LET-23 overexpression rescues the lin-2 or lin-7 vulvaless phenotype, suggesting that increased receptor density can compensate for mislocalization. These results suggest that proper localization of LET-23 receptor to the Pn.p cell junctions is required for signaling activity.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Caenorhabditis elegans / genetics*
  • Caenorhabditis elegans Proteins*
  • Cloning, Molecular
  • Embryonic Induction / genetics
  • Epithelium / chemistry
  • Epithelium / physiology
  • Epithelium / ultrastructure
  • ErbB Receptors / genetics*
  • Female
  • Genes, Helminth / physiology
  • Helminth Proteins / genetics*
  • Helminth Proteins / physiology
  • Intercellular Junctions / chemistry*
  • Membrane Proteins / genetics*
  • Membrane Proteins / physiology
  • Molecular Sequence Data
  • Mutation / physiology
  • Phenotype
  • Protein Structure, Tertiary
  • Proteins*
  • Sequence Homology, Amino Acid
  • Signal Transduction / genetics
  • Vulva / cytology
  • Vulva / growth & development
  • Vulva / physiology


  • Caenorhabditis elegans Proteins
  • Helminth Proteins
  • LIN-7 protein, C elegans
  • Lin-2 protein, C elegans
  • Membrane Proteins
  • Proteins
  • lin-10 protein, C elegans
  • ErbB Receptors
  • let-23 protein, C elegans

Associated data

  • GENBANK/U78092