The Transition of pre-BI to pre-BII Cells Is Dependent on the VH Structure of the mu/surrogate L Chain Receptor

EMBO J. 1996 Apr 1;15(7):1524-33.


We have demonstrated previously that the majority ( > 90%) of VH12 B cells are absent from the adult peripheral repertoire, and that most that remain have the fourth position at the D-J function (designated 10/G4). We report here that most VH 12-expressing pre-B cells are lost during the transition from the pre-BI to the pre-BII cell stage in normal mice, and that pre-BII cell productive (P) rearrangements ar enriched in 10/G4 CDR3. This coincides with the initial expression of H chain and the generation of the mu/surrogate L chain (SL) receptor. In contrast, there is not enrichment for 10/G4 CDR3 in mu MT mice, and the frequency of P rearrangements is as expected from a random rearrangement mechanism, ruling out a biased rearrangement mechanism unique to VH12. We have also demonstrated that non-10/G4 mu chains can associate with SL and be expressed on the cell surface, suggesting that they are available on the cell surface for selection. Thus, transition of pre-BI to pre-BII cells is dependent on the structure of the VH domain.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibody Diversity
  • B-Lymphocytes / cytology
  • B-Lymphocytes / immunology*
  • Base Sequence
  • Cell Differentiation / genetics
  • Cell Differentiation / immunology
  • DNA / genetics
  • DNA Primers / genetics
  • Gene Rearrangement, B-Lymphocyte*
  • Gene Rearrangement, B-Lymphocyte, Heavy Chain
  • Gene Rearrangement, B-Lymphocyte, Light Chain
  • Hematopoietic Stem Cells / cytology
  • Hematopoietic Stem Cells / immunology
  • Immunoglobulin Heavy Chains / genetics
  • Immunoglobulin Light Chains / genetics
  • Immunoglobulin Light Chains, Surrogate
  • Immunoglobulin Variable Region / genetics
  • Immunoglobulin mu-Chains / genetics
  • Membrane Glycoproteins / genetics
  • Mice
  • Mice, Knockout
  • Molecular Sequence Data
  • Transfection


  • DNA Primers
  • Immunoglobulin Heavy Chains
  • Immunoglobulin Light Chains
  • Immunoglobulin Light Chains, Surrogate
  • Immunoglobulin Variable Region
  • Immunoglobulin mu-Chains
  • Membrane Glycoproteins
  • DNA