Comparative study of the sugar chains of alkaline phosphatases purified from rat liver and rat AH-130 hepatoma cells. Occurrence of fucosylated high-mannose-type and hybrid-type sugar chains

Eur J Biochem. 1996 Mar 1;236(2):579-90. doi: 10.1111/j.1432-1033.1996.t01-1-00579.x.


The N-linked sugar chains of alkaline phosphatases, purified from rat AH-130 hepatoma and from normal rat liver, were released quantitatively as oligosaccharides by hydrazinolysis and were labeled by reduction with NaB3H4. A comparative study of their structures revealed that following structural differences are induced by hepatocyte carcinogenesis: complex-type tetraantennary sugar chains and hybrid-type sugar chains appear; outer-chain moieties of the sugar chains of the hepatoma enzyme contain exclusively the Gal(Beta 1-4)GlcNAc groups (type 2 chains) but those of the normal enzyme contain other Gal(Beta 1-)GlcNAc groups and type 2 chains; and novel fucosylated high-mannose-type sugar chains are found in the oligosaccharides of the hepatoma enzyme.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkaline Phosphatase / chemistry*
  • Animals
  • Carbohydrate Sequence
  • Glycoproteins / chemistry*
  • Liver / enzymology*
  • Liver Neoplasms, Experimental / enzymology*
  • Molecular Sequence Data
  • Oligosaccharides / chemistry
  • Oxidation-Reduction
  • Periodic Acid / chemistry
  • Rats


  • Glycoproteins
  • Oligosaccharides
  • Periodic Acid
  • Alkaline Phosphatase