Fibroblast growth factor-1-inducible gene FR-17 encodes a nonmuscle alpha-actinin isoform

J Cell Physiol. 1996 May;167(2):261-8. doi: 10.1002/(SICI)1097-4652(199605)167:2<261::AID-JCP9>3.0.CO;2-N.

Abstract

Polypeptide growth factor binding to cell surface receptors activates a cytoplasmic signaling cascade that ultimately promotes the expression of specific nuclear genes. As an approach to investigate the molecular mechanism of fibroblast growth factor (FGF)-1 mitogenic signaling, we have begun to identify and characterize FGF-1-inducible genes in murine NIH 3T3 cells. Here we report that one of these genes, termed FGF-regulated (FR)-17, is predicted to encode a nonmuscle isoform of alpha-actinin, an actin cross-linking protein found along microfilaments and in focal adhesion plaques. FGF-1 induction of alpha-actinin mRNA expression is first detectable at 2 h after mitogen addition and is dependent on the novo RNA and protein synthesis. Maximal alpha-actinin mRNA expression, corresponding to an approximately nineteenfold level of induction, is present after 12 h of FGF-1 stimulation. Western blot analysis indicated that FGF-1-stimulated cells also produce an increased amount of alpha-actinin protein. The FGF-1-related mitogen FGF-2, calf serum, several of the polypeptide growth factors present in serum, and the tumor promoter phorbol myristate acetate can also induce alpha-actinin mRNA expression. Finally, nonmuscle alpha-actinin mRNA is expressed in vivo in a tissue-specific manner, with relatively high levels detected in adult mouse intestine and kidney. These results indicate that nonmuscle alpha-actinin is a serum-, polypeptide growth factor-, and tumor promoter-inducible gene in mouse fibroblasts.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 3T3 Cells / cytology
  • 3T3 Cells / physiology
  • Actinin / chemistry
  • Actinin / genetics*
  • Animals
  • Base Sequence
  • Blood Proteins / pharmacology
  • Cell Adhesion / drug effects
  • Cell Cycle / drug effects
  • Cloning, Molecular
  • DNA, Complementary / analysis
  • Fibroblast Growth Factor 1 / pharmacology*
  • Gene Expression Regulation / drug effects
  • Growth Substances / pharmacology
  • Isomerism
  • Mice
  • Molecular Sequence Data
  • RNA, Messenger / analysis
  • Sequence Homology, Amino Acid
  • Tetradecanoylphorbol Acetate / pharmacology
  • Time Factors

Substances

  • Blood Proteins
  • DNA, Complementary
  • Growth Substances
  • RNA, Messenger
  • Fibroblast Growth Factor 1
  • Actinin
  • Tetradecanoylphorbol Acetate

Associated data

  • GENBANK/U41415
  • GENBANK/U41416