Abstract
Two full-length cDNAs encoding hydroxypyruvate reductase were isolated from a cDNA library constructed with poly(A)+ RNA from pumpkin green cotyledons. One of the cDNAs, designated HPR1, encodes a polypeptide of 386 amino acids, while the other cDNA, HPR2 encodes a polypeptide of 381 amino acids. Although the nucleotide and deduced amino acid sequences of these cDNAs are almost identical, the deduced HPR1 protein contains Ser-Lys-Leu at its carboxy-terminal end, which is known as a microbody-targeting signal, while the deduced HPR2 protein does not. Analysis of genomic DNA strongly suggests that HPR1 and HPR2 are produced by alternative splicing.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Alcohol Oxidoreductases / genetics*
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Alternative Splicing
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Amino Acid Sequence
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Base Sequence
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Biological Transport
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Cell Compartmentation*
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Cotyledon / enzymology
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DNA, Complementary / genetics
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Gene Library
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Hydroxypyruvate Reductase
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Isoenzymes / genetics*
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Microbodies / enzymology*
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Molecular Sequence Data
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Plant Proteins / genetics
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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Vegetables / enzymology
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Vegetables / genetics*
Substances
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DNA, Complementary
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Isoenzymes
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Plant Proteins
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Alcohol Oxidoreductases
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Hydroxypyruvate Reductase
Associated data
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GENBANK/D49431
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GENBANK/D49432
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GENBANK/D49433