Structure and characterization of a putative drought-inducible H1 histone gene

Plant Mol Biol. 1996 Jan;30(2):255-68. doi: 10.1007/BF00020112.

Abstract

A drought- and abscisic acid (ABA)-inducible gene, His1, was isolated from Lycopersicon pennellii, a drought-resistant relative of cultivated tomato, and the gene structure was defined experimentally. The nucleotide sequence of His1 predicts a protein of 202 amino acid residues, with a significant sequence homology to plant H1 histones. Consensus sequences for both H1 histone-specific promoter elements as well as an ABA-responsive element were identified in the 5'-flanking region of His1. Transcripts of this gene accumulate in leaf tissue in response to drought in three tomato species including cultivated tomato (L. esculentum), L. pennellii, and L. chilense, as well as in tobacco. Transcripts for His1 are constitutively expressed in roots; transcript abundances in tomato root tips were equivalent to transcript abundances in more mature regions of the seedling root. The accumulation in leaves of transcripts for His1 preceded visible symptoms of drought stress in the plants. Transcript accumulation was detected in both drought-sensitive and drought-resistant species at similar leaf water potentials, psi W -1.3 to -1.4 MPa.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Abscisic Acid / pharmacology
  • Amino Acid Sequence
  • Base Sequence
  • Disasters
  • Gene Expression Regulation, Plant*
  • Histones / biosynthesis
  • Histones / genetics*
  • Molecular Sequence Data
  • Plant Roots / metabolism
  • Polymerase Chain Reaction
  • RNA, Messenger / analysis
  • Regulatory Sequences, Nucleic Acid
  • Sequence Analysis, DNA
  • Sequence Homology
  • Solanum lycopersicum / drug effects
  • Solanum lycopersicum / genetics*
  • Species Specificity
  • Tissue Distribution
  • Water / physiology

Substances

  • Histones
  • RNA, Messenger
  • Water
  • Abscisic Acid

Associated data

  • GENBANK/U01890