Evidence for a new step in telomere maintenance

Cell. 1996 May 3;85(3):423-33. doi: 10.1016/s0092-8674(00)81120-4.


The strand of telomeric DNA that runs 5'-3' toward a chromosome end is typically G rich. Telomerase-generated G tails are expected at one end of individual DNA molecules. Saccharomyces telomeres acquire TG1-3 tails late in S phase. Moreover, the telomeres of linear plasmids can interact when the TG1-3 tails are present. Molecules that mimic the structures predicted for telomere replication intermediates were generated in vitro. These in vitro generated molecules formed telomere-telomere interactions similar to those on molecules isolated from yeast, but only if both ends that interacted had a TG1-3 tail. Moreover, TG1-3 tails were generated in vivo in cells lacking telomerase. These data suggest a new step in telomere maintenance, cell cycle-regulated degradation of the C1-3A strand, which can generate a potential substrate for telomerase and telomere-binding proteins at every telomere.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Cations / pharmacology
  • Cell Cycle / genetics
  • DNA Replication / genetics
  • DNA, Fungal / genetics
  • Hot Temperature
  • Magnesium / pharmacology
  • Molecular Sequence Data
  • Plasmids
  • RNA, Fungal / genetics
  • Repetitive Sequences, Nucleic Acid
  • Saccharomyces cerevisiae / cytology
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae / genetics
  • Telomerase / genetics
  • Telomere / enzymology*
  • Telomere / genetics*


  • Cations
  • DNA, Fungal
  • RNA, Fungal
  • Telomerase
  • Magnesium