Nested-polymerase chain reaction for the detection of Helicobacter pylori infection with novel primers designed by sequence analysis of urease A gene in clinically isolated bacterial strains

Biochem Biophys Res Commun. 1996 Feb 6;219(1):266-72. doi: 10.1006/bbrc.1996.0216.


We have established a highly sensitive semi-nested PCR assay for the detection of H. pylori infection using gastric juice samples, which can be aspirated with disposable nasogastric tubes. The primers targeting H. pylori urease A gene were designed based on the sequence conservation analysis of sixteen H. pylori strains isolated from Japanese patients. The efficacy of the PCR assay, designated as the URA-PCR, was confirmed by in vitro and in vivo assessments. Its sensitivity was 97.5% in the gastric juice samples aspirated from forty patients with proven H. pylori infection, and was significantly higher than that obtained with previously described PCR assays.

Publication types

  • Comparative Study

MeSH terms

  • Base Sequence
  • DNA Primers
  • Gastric Juice / microbiology
  • Genes, Bacterial*
  • Helicobacter Infections / diagnosis*
  • Helicobacter pylori* / genetics
  • Helicobacter pylori* / isolation & purification
  • Humans
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods*
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Sequence Homology, Nucleic Acid
  • Urease / genetics*


  • DNA Primers
  • Urease

Associated data

  • GENBANK/M60398