Vasopressor activities of N-terminal fragments of adrenomedullin in anesthetized rat

Biochem Biophys Res Commun. 1996 Feb 6;219(1):59-63. doi: 10.1006/bbrc.1996.0181.

Abstract

Adrenomedullin (AM) is a vasorelaxant peptide that was recently isolated from human pheochromocytoma. In contrast to human (h) AM, which has vasodepressor activity, a synthetic N-terminal fragment of hAM, hAM-(1-25)-NH2 showed vasopressor activity in the anesthetized rat. The N-terminal peptides hAM-1-31)-NH2, hAM-(1-25)-OH, hAM-(1-21)-NH2, acetyl-hAM-(16-21)-NH2, and acetyl-hAM-(16-36)-OH all showed vasopressor activities. The potency of hAM-(1-21)-NH2, acetyl-hAM-(16-21)-NH2 was greater than that of hAM-(1-25)-NH2. Pretreatment with phenoxybenzamine, guanethidine, or reserpine attenuated vasopressor activities of these peptides. These data suggested that vasopressor activity of N-terminal fragment of hAM is due to a stimulation of endogenous catecholamine release.

Publication types

  • Comparative Study

MeSH terms

  • Adrenal Gland Neoplasms / chemistry
  • Adrenomedullin
  • Anesthesia, General
  • Animals
  • Blood Pressure / drug effects*
  • Consciousness
  • Guanethidine / pharmacology
  • Humans
  • Male
  • Peptide Fragments / chemical synthesis
  • Peptide Fragments / pharmacology*
  • Peptides / isolation & purification
  • Peptides / pharmacology*
  • Phenoxybenzamine / pharmacology
  • Pheochromocytoma / chemistry
  • Rats
  • Rats, Sprague-Dawley
  • Reserpine / pharmacology
  • Structure-Activity Relationship
  • Vasodilator Agents / pharmacology*

Substances

  • Peptide Fragments
  • Peptides
  • Vasodilator Agents
  • Phenoxybenzamine
  • Adrenomedullin
  • Reserpine
  • Guanethidine