Schizosaccharomyces pombe rad23 is allelic with swi10, a mating-type switching/radioresistance gene that shares sequence homology with human and mouse ERCC1

Gene. 1996 Apr 17;170(1):113-7. doi: 10.1016/0378-1119(95)00872-1.


Schizosaccharomyces pombe (Sp) rad23-1 mutant cells are extremely sensitive to UV light and ionizing radiation. A genomic DNA fragment that contains wild-type (wt) rad23 has been cloned. The DNA sequence of this cloned gene has been determined and was found to be identical to the previously characterized mating-type switching/radioresistance gene, swi10. Complementation tests between rad23-1 and swi10-154 mutant cells exclusively produce UV-sensitive progeny and confirm that these two genes are allelic. The DNA sequences of rad23-1 and swi10-154 reveal that each contains a single, unique point mutation. In rad23-1, Glu231 changes to a stop codon, resulting in the production of a truncated protein. In swi10-154, a G to A transition mutation is within a splice consensus sequence for intron 1. Therefore, the corresponding mRNA is incapable of being processed appropriately.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alleles*
  • Animals
  • Base Sequence
  • Codon / genetics
  • DNA Mutational Analysis
  • DNA Repair
  • DNA, Fungal / genetics
  • DNA-Binding Proteins / genetics*
  • Endonucleases*
  • Fungal Proteins / genetics*
  • Humans
  • Mice
  • Molecular Sequence Data
  • Mutation
  • Proteins / genetics*
  • Radiation Tolerance / genetics
  • Schizosaccharomyces / genetics*
  • Schizosaccharomyces / radiation effects
  • Schizosaccharomyces pombe Proteins*
  • Sequence Homology, Nucleic Acid
  • Species Specificity
  • Ultraviolet Rays


  • Codon
  • DNA, Fungal
  • DNA-Binding Proteins
  • Fungal Proteins
  • Proteins
  • RHP23 protein, S pombe
  • SWI10 protein, S pombe
  • Schizosaccharomyces pombe Proteins
  • ERCC1 protein, human
  • Endonucleases
  • Ercc1 protein, mouse