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Construction of an Alkaline Phosphatase Fusion-Generating Transposon, mTn10phoA

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Construction of an Alkaline Phosphatase Fusion-Generating Transposon, mTn10phoA

M S McClain et al. Gene.

Abstract

We constructed a derivative of the mini-transposon mTn10 that generates translational fusions to the phoA gene from Escherichia coli and carries the KmR determinant from Tn5. This new transposon, mTn10phoA, is carried on a mobilizable plasmid with both selectable and counterselectable markers. The plasmid carrying mTn10phoA was introduced into Legionella pneumophila. Southern hybridization analysis indicated that the mTn10phoA insertions were randomly distributed.

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