Demonstration of the oligoclonality of an enteropathy associated T-cell lymphoma by monoclonal antibodies and PCR analysis of the T-cell receptor V-beta repertoire on fixed tissue

Hum Pathol. 1996 May;27(5):509-13. doi: 10.1016/s0046-8177(96)90095-7.

Abstract

The apparent clonality of T cells present in enteropathy associated T cell lymphomas (EATCLs) has been previously reported by showing T cell receptor (TCR) gene rearrangement in fresh tumor tissue. The EATCL presented here exhibits the novel phenotype CD3+, HML-1+, CD4+, CD8+, and TCR Vbeta 8+. The oligoclonality of the tumor cells is shown using the polymerase chain reaction (PCR) on cDNA from RNA extracted from formalin-fixed paraffin-embedded tissue. The T cells present in the lymphoma were predominantly TCR Vbeta 8+.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Antibodies, Monoclonal
  • Female
  • Gene Rearrangement
  • Humans
  • Ileal Neoplasms / genetics
  • Ileal Neoplasms / pathology*
  • Lymphoma, T-Cell / genetics
  • Lymphoma, T-Cell / pathology*
  • Polymerase Chain Reaction
  • Receptors, Antigen, T-Cell / genetics*
  • T-Lymphocyte Subsets*

Substances

  • Antibodies, Monoclonal
  • Receptors, Antigen, T-Cell