Inversely correlated expression of p16 and Rb protein in non-small cell lung cancers: an immunohistochemical study

Int J Cancer. 1996 Feb 8;65(4):442-5. doi: 10.1002/(SICI)1097-0215(19960208)65:4<442::AID-IJC8>3.0.CO;2-X.


Cdk4-mediated phosphorylation of Rb protein is inhibited by p16, a product of a possible tumor suppressor gene. We examined the expression of p16 and Rb protein by means of immunohistochemistry in 61 non-small cell lung cancers and have demonstrated an inverse relationship between the expression of p16 and Rb protein: 28/30 specimens that did not stain for p16 stained for Rb and 21/31 p16-positive specimens did not stain for Rb. Only 1 of the p16-negative specimens had a mutation of exon 2 of the CDKN2 gene. Our results indirectly support the theory that p16 expression is negatively regulated by the functional Rb protein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Carcinoma, Non-Small-Cell Lung / chemistry*
  • Carcinoma, Non-Small-Cell Lung / genetics
  • Carrier Proteins / analysis*
  • Carrier Proteins / genetics
  • Cyclin-Dependent Kinase Inhibitor p16
  • Female
  • Genes, Tumor Suppressor
  • Humans
  • Immunohistochemistry
  • Lung Neoplasms / chemistry*
  • Lung Neoplasms / genetics
  • Male
  • Molecular Sequence Data
  • Mutation
  • Retinoblastoma Protein / analysis*


  • Carrier Proteins
  • Cyclin-Dependent Kinase Inhibitor p16
  • Retinoblastoma Protein