T lymphocyte recruitment by interleukin-8 (IL-8). IL-8-induced degranulation of neutrophils releases potent chemoattractants for human T lymphocytes both in vitro and in vivo

J Clin Invest. 1996 Apr 15;97(8):1931-41. doi: 10.1172/JCI118625.


IL-8 has been shown to be a human neutrophil and T cell chemoattractant in vitro. In an effort to assess the in vivo effects of IL-8 on human leukocyte migration, we examined the ability of rhIL-8 to induce human T cell infiltration using a human/mouse model in which SCID mice were administered human peripheral blood lymphocytes intraperitoneally, followed by subcutaneous injections of rhIL-8. rhIL-8 induced predominantly murine neutrophil accumulation by 4 h after administration while recombinant human macrophage inflammatory protein-1beta (rhMIP-1beta) induced both murine monocytes and human T cell infiltration during the same time period as determined by immunohistology. Interestingly, 72 h after chemokine administration, a marked human T cell infiltrate was observed in the IL-8 injection site suggesting that rhIL-8 may be acting indirectly possibly through a murine neutrophil-derived T cell chemoattractant. This hypothesis was confirmed using granulocyte-depleted SCID mice. Moreover, human neutrophils stimulated in vitro with IL-8 were found to release granule-derived factor(s) that induce in vitro T cell and monocyte chemotaxis and chemokinesis. This T cell and monocyte chemotactic activity was detected in extracts of both azurophilic and specific granules. Together, these results demonstrate that neutrophils store and release, upon stimulation with IL-8 or other neutrophil activators, chemoattractants that mediate T cell and monocyte accumulation at sites of inflammation.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Chemokine CCL4
  • Chemotaxis, Leukocyte*
  • Cytoplasmic Granules / physiology
  • Cytoplasmic Granules / ultrastructure
  • Growth Inhibitors / pharmacology
  • Humans
  • Interleukin-8 / pharmacology*
  • Kinetics
  • Macrophage Inflammatory Proteins
  • Mice
  • Mice, SCID
  • Monocytes / physiology
  • Monokines / pharmacology
  • N-Formylmethionine Leucyl-Phenylalanine / pharmacology
  • Neutrophils / drug effects
  • Neutrophils / physiology*
  • Neutrophils / ultrastructure
  • Recombinant Proteins / pharmacology
  • T-Lymphocytes / drug effects
  • T-Lymphocytes / immunology*
  • T-Lymphocytes / physiology
  • Time Factors


  • Chemokine CCL4
  • Growth Inhibitors
  • Interleukin-8
  • Macrophage Inflammatory Proteins
  • Monokines
  • Recombinant Proteins
  • N-Formylmethionine Leucyl-Phenylalanine