Deletion of glycoprotein gE reduces the propagation of pseudorabies virus in the nervous system of mice after intranasal inoculation

Virology. 1996 May 1;219(1):279-84. doi: 10.1006/viro.1996.0247.


A pseudorabies virus (PrV) mutant, deficient in the nonessential glycoprotein E (gE) and expressing the LacZ gene (gE- beta gal+ PrV), and its rescued virus were inoculated intranasally in mice. The median lethal dose of gE- beta gal+ PrV was similar to that of the parental Kaplan strain, but mice survived longer and did not develop symptoms of pseudorabies. In the nasal mucosa, gE- beta gal+ PrV replicated less efficiently than rescued virus. gE- beta gal+ PrV could infect first-order trigeminal and sympathetic neurons innervating the nasal mucosa. However, transneuronal transfer to second-order cells groups did not occur in trigeminal pathways and was severely reduced in sympathetic pathways. The mutant was also unable to propagate in the parasympathetic system. In contrast, gE-rescued virus was transferred transneuronally in trigeminal, sympathetic, and parasympathetic pathways, like wild-type PrV. These findings provide further evidence that deletion of gE specifically affects transneuronal transfer of PrV more than penetration and multiplication of the virus in first-order neurons.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Intranasal
  • Animals
  • Chlorocebus aethiops
  • Disease Models, Animal
  • Female
  • Gene Deletion
  • Herpesvirus 1, Suid / genetics
  • Herpesvirus 1, Suid / physiology*
  • Mice
  • Nasal Cavity / virology
  • Neurons / virology*
  • Olfactory Pathways / cytology
  • Olfactory Pathways / virology
  • Parasympathetic Nervous System / virology
  • Pseudorabies / virology*
  • Respiratory System / cytology
  • Respiratory System / virology
  • Sympathetic Nervous System / virology
  • Trigeminal Nerve / virology
  • Vero Cells
  • Viral Envelope Proteins / genetics
  • Viral Envelope Proteins / physiology*
  • Virus Replication


  • Viral Envelope Proteins
  • glycoprotein E, Suid herpesvirus 1