Changes in cell growth, cyclin/kinase, endogenous phosphoproteins and nm23 gene expression in human prostatic JCA-1 cells treated with modified citrus pectin

Biochem Mol Biol Int. 1995 Nov;37(5):833-41.

Abstract

Modified citrus pectin (MCP) added to the media of cultured androgen-independent human prostatic JCA-1 cells reduced cell growth and correspondingly [3H]thymidine incorporation into DNA, which was correlated with the down-regulation of cyclin B and p34cdc2 MCP also induced distinct increases in specific endogenous phosphoproteins, including a cAMP-stimulated 52,000 (52-kDa) protein. Since metastatis has been inversely correlated with nm23 gene expression in some cancer cells and was reportedly inhibited by MCP in a rat prostate model, we investigated steady state level changes in the nm23 protein in JCA-1 cells and found it to be unexpectedly suppressed as a result of exposure to MCP.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CDC2 Protein Kinase / drug effects*
  • CDC2 Protein Kinase / metabolism
  • Cell Division / drug effects
  • Citrus / chemistry
  • Cyclin D
  • Cyclin-Dependent Kinase 4
  • Cyclin-Dependent Kinases / metabolism
  • Cyclins / drug effects*
  • Cyclins / metabolism
  • Gene Expression Regulation, Neoplastic / drug effects*
  • Humans
  • Male
  • Neoplasm Metastasis / genetics
  • Pectins / pharmacology*
  • Phosphoproteins / drug effects*
  • Phosphoproteins / metabolism
  • Prostatic Neoplasms / drug therapy*
  • Prostatic Neoplasms / metabolism
  • Prostatic Neoplasms / pathology
  • Proto-Oncogene Proteins*
  • Rats
  • Tumor Cells, Cultured
  • Tumor Suppressor Protein p53 / metabolism

Substances

  • Cyclin D
  • Cyclins
  • Phosphoproteins
  • Proto-Oncogene Proteins
  • Tumor Suppressor Protein p53
  • Pectins
  • CDC2 Protein Kinase
  • CDK4 protein, human
  • Cdk4 protein, rat
  • Cyclin-Dependent Kinase 4
  • Cyclin-Dependent Kinases