Abstract
Modified citrus pectin (MCP) added to the media of cultured androgen-independent human prostatic JCA-1 cells reduced cell growth and correspondingly [3H]thymidine incorporation into DNA, which was correlated with the down-regulation of cyclin B and p34cdc2 MCP also induced distinct increases in specific endogenous phosphoproteins, including a cAMP-stimulated 52,000 (52-kDa) protein. Since metastatis has been inversely correlated with nm23 gene expression in some cancer cells and was reportedly inhibited by MCP in a rat prostate model, we investigated steady state level changes in the nm23 protein in JCA-1 cells and found it to be unexpectedly suppressed as a result of exposure to MCP.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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CDC2 Protein Kinase / drug effects*
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CDC2 Protein Kinase / metabolism
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Cell Division / drug effects
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Citrus / chemistry
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Cyclin D
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Cyclin-Dependent Kinase 4
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Cyclin-Dependent Kinases / metabolism
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Cyclins / drug effects*
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Cyclins / metabolism
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Gene Expression Regulation, Neoplastic / drug effects*
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Humans
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Male
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Neoplasm Metastasis / genetics
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Pectins / pharmacology*
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Phosphoproteins / drug effects*
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Phosphoproteins / metabolism
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Prostatic Neoplasms / drug therapy*
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Prostatic Neoplasms / metabolism
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Prostatic Neoplasms / pathology
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Proto-Oncogene Proteins*
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Rats
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Tumor Cells, Cultured
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Tumor Suppressor Protein p53 / metabolism
Substances
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Cyclin D
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Cyclins
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Phosphoproteins
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Proto-Oncogene Proteins
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Tumor Suppressor Protein p53
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Pectins
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CDC2 Protein Kinase
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CDK4 protein, human
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Cdk4 protein, rat
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Cyclin-Dependent Kinase 4
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Cyclin-Dependent Kinases