Selective transport of blood-borne interleukin-1 alpha into the posterior division of the septum of the mouse brain

Brain Res. 1995 Nov 27;700(1-2):83-8. doi: 10.1016/0006-8993(95)00913-b.

Abstract

Film autoradiography was used to demonstrate the transport and sites of accumulation of blood-borne radioiodinated interleukin-1 alpha (II-1 alpha) and other cytokines into the brain after intravenous administration. [125 I]Il-1 alpha, [125I]Il-1 beta, [125I]interleukin-1 receptor antagonist (II-1ra), and [125I]tumor necrosis factor-alpha (TNF alpha) labeled the choroid plexus and the capillary network 30 min after injection into the blood, suggesting that these areas may serve as sites of blood-to-brain transport. [125I]Il-1alpha, but not [125I]Il-1beta, [125I]Il-1ra, [125I]TNF alpha, or [125I]interleukin-2 (Il-2), was also found localized to the caudal region of the septal nuclei. Only unlabeled II-1 alpha was able to inhibit this accumulation. These findings provide further evidence for the passage of select cytokines across the blood-brain barrier (BBB) and are the first to identify a target site within the central nervous system (CNS) for a transported cytokine.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Autoradiography
  • Blood-Brain Barrier / physiology*
  • Central Nervous System / metabolism*
  • Cytokines / metabolism*
  • Interleukin-1 / blood
  • Interleukin-1 / pharmacokinetics*
  • Male
  • Mice
  • Mice, Inbred ICR
  • Septal Nuclei / metabolism*

Substances

  • Cytokines
  • Interleukin-1