Asymmetric Accumulation of Ash1p in Postanaphase Nuclei Depends on a Myosin and Restricts Yeast Mating-Type Switching to Mother Cells

Cell. 1996 Mar 8;84(5):699-709. doi: 10.1016/s0092-8674(00)81048-x.

Abstract

Cell division in haploid yeast gives rise to a "mother" cell capable of mating-type switching and a "daughter" cell that is not. Switching is initiated by the HO endonuclease, whose gene is only transcribed in cells that have previously given birth to a bud (mother cells). HO expression depends on a minimyosin, She1p/Myo4p, which accumulates preferentially in growing buds. We describe a gene, ASH1, that is necessary to repress HO in daughters. ASH1 encodes a zinc finger protein whose preferential accumulation in daughter cell nuclei at the end of anaphase depends on She1p/Myo4p. The greater abundance of Ash1p in daughter cells is responsible for restricting HO expression to mother cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Anaphase
  • Base Sequence
  • Cell Nucleus / metabolism*
  • Cloning, Molecular
  • Crosses, Genetic
  • DNA-Binding Proteins*
  • Deoxyribonucleases, Type II Site-Specific / biosynthesis*
  • Deoxyribonucleases, Type II Site-Specific / genetics
  • Epitopes / analysis
  • Fungal Proteins / biosynthesis
  • Fungal Proteins / metabolism*
  • Gene Expression
  • Genes, Fungal*
  • Genes, Mating Type, Fungal*
  • Molecular Sequence Data
  • Mutagenesis
  • Myosin Heavy Chains*
  • Myosin Type V*
  • Myosins / biosynthesis
  • Myosins / metabolism*
  • Open Reading Frames
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / chemistry
  • Repressor Proteins*
  • Saccharomyces cerevisiae / cytology*
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / physiology*
  • Saccharomyces cerevisiae Proteins*
  • Sequence Homology, Amino Acid
  • Species Specificity
  • Transcription Factors / biosynthesis*
  • Transcription Factors / chemistry
  • Transcription Factors / genetics
  • Transcription, Genetic
  • Zinc Fingers

Substances

  • ASH1 protein, S cerevisiae
  • DNA-Binding Proteins
  • Epitopes
  • Fungal Proteins
  • Myo4 protein, S cerevisiae
  • Recombinant Proteins
  • Repressor Proteins
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • HO protein, S cerevisiae
  • SCEI protein, S cerevisiae
  • Deoxyribonucleases, Type II Site-Specific
  • Myosin Type V
  • Myosin Heavy Chains
  • Myosins