Abstract
The abundance of macrophages in localized cutaneous leishmaniasis (LCL) and diffuse cutaneous leishmaniasis (DCL) lesions and differences in the composition of T cell subsets indicate involvement of cell-specific chemotaxis processes. The expression of macrophage chemoattractant protein (MCP)-1, macrophage inflammatory protein (MIP)-1 alpha and -1 beta, RANTES (regulated on activation, normal T cell expressed and secreted), I-309, and interleukin-8 were investigated in lesions of patients with LCL or DCL. In LCL, high levels of MCP-1 and moderate levels of MIP-1 alpha were detected. In DCL, MCP-1 expression was significantly lower and MIP-1 alpha expression was predominant. All other chemokines investigated were minimally expressed or absent. These findings suggest that MCP-1 and MIP-alpha are responsible for the recruitment of macrophages and T cells in cutaneous leishmaniasis. The results show that self-healing LCL is associated with higher levels of MCP-1, which may stimulate macrophage microbicidal mechanisms, and nonhealing DCL is associated with higher levels of MIP-alpha.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Chemokine CCL1
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Chemokine CCL2 / metabolism
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Chemokine CCL4
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Chemokine CCL5 / metabolism
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Chemokines / metabolism*
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Chemokines, CC*
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Chemotaxis
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Cytokines / metabolism
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DNA Primers / genetics
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DNA, Protozoan / genetics
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DNA, Protozoan / isolation & purification
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Humans
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Immunohistochemistry
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In Situ Hybridization
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Interleukin-8 / metabolism
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Leishmania mexicana / genetics
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Leishmania mexicana / isolation & purification
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Leishmaniasis, Cutaneous / immunology*
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Leishmaniasis, Cutaneous / parasitology
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Leishmaniasis, Cutaneous / pathology
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Leishmaniasis, Diffuse Cutaneous / immunology*
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Leishmaniasis, Diffuse Cutaneous / parasitology
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Leishmaniasis, Diffuse Cutaneous / pathology
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Macrophage Inflammatory Proteins
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Macrophages / immunology
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Molecular Sequence Data
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Monokines / metabolism
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T-Lymphocytes / immunology
Substances
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CCL1 protein, human
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Chemokine CCL1
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Chemokine CCL2
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Chemokine CCL4
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Chemokine CCL5
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Chemokines
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Chemokines, CC
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Cytokines
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DNA Primers
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DNA, Protozoan
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Interleukin-8
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Macrophage Inflammatory Proteins
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Monokines