Neutralization of Human Cytomegalovirus by Human CD13-specific Antibodies

J Infect Dis. 1996 May;173(5):1062-71. doi: 10.1093/infdis/173.5.1062.

Abstract

The mechanisms of inhibition of cytomegalovirus (CMV) infection by human CD13 (aminopeptidase N)-specific antibodies were studied. These antibodies protect CD13-negative and -positive cells from CMV infection only if incubated with the virus inoculum, suggesting they bind to CMV virions. The association of a CD13-like molecule with virions was further supported by the transfer of CD13 immunoreactivity to the surface of CD13-negative cells upon binding of CMV; the binding of CD13-specific antibodies directly to the surface of CMV virions; and the presence of anti-CD13 immunoreactive bands, including one with mobility similar to that of native cellular CD13 on immunoblots of proteins of purified CMV particles. Importantly, CD13-specific antibodies neutralize CMV in urine of neonates with congenital CMV, indicating that the CD13-like molecule is associated with natural CMV and not acquired in vitro. These studies demonstrate that a CD13-like molecule is associated with CMV particles and may be important in CMV pathogenesis.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology*
  • Antibodies, Viral / immunology*
  • Antibody Specificity
  • CD13 Antigens / immunology*
  • Cell Line
  • Cross Reactions
  • Cytomegalovirus / immunology*
  • Cytomegalovirus Infections / congenital
  • Cytomegalovirus Infections / immunology
  • Cytomegalovirus Infections / virology
  • Epitopes / analysis
  • Female
  • Humans
  • Infant, Newborn
  • Mice
  • Neutralization Tests
  • Virion / metabolism

Substances

  • Antibodies, Monoclonal
  • Antibodies, Viral
  • Epitopes
  • CD13 Antigens