A noncovalent complex vaccine prepared with detoxified Escherichia coli J5 (Rc chemotype) lipopolysaccharide and Neisseria meningitidis Group B outer membrane protein produces protective antibodies against gram-negative bacteremia

J Infect Dis. 1996 May;173(5):1157-63. doi: 10.1093/infdis/173.5.1157.


Earlier studies showed that purified IgG from sera of rabbits immunized with a boiled Escherichia coli J5 (Rc chemotype) whole cell vaccine protected neutropenic rats against gram-negative bacterial sepsis. In the present study, de-O-acylated J5 lipopolysaccharide (J5 DLPS) as a noncovalent complex with Neisseria meningitidis group B outer membrane protein (GBOMP) elicited anti-J5 LPS antibodies in rabbits. IgG prepared from immune rabbit sera protected neutropenic rats against lethal challenge with Pseudomonas aeruginosa 12:4:4 (Fisher Devlin immunotype 6). Sixteen of 26 rats treated with the postimmune serum IgG were protected compared with none of 20 rats treated with the control rabbit serum IgG (P < .001). In vitro binding studies showed binding of anti-J5 IgG to several gram-negative bacteria. These results indicate that a subunit vaccine made of J5 DLPS as a noncovalent complex with GBOMP may protect against gram-negative bacteremia.

MeSH terms

  • Animals
  • Antibodies, Bacterial / blood
  • Antibodies, Bacterial / immunology
  • Antibody Specificity
  • Bacteremia / immunology
  • Bacteremia / prevention & control*
  • Bacterial Outer Membrane Proteins / immunology*
  • Bacterial Vaccines / immunology*
  • Endotoxins / blood
  • Escherichia coli / immunology*
  • Escherichia coli Infections / immunology
  • Escherichia coli Vaccines
  • Female
  • Gram-Negative Bacterial Infections / immunology
  • Gram-Negative Bacterial Infections / prevention & control*
  • Immune Sera / immunology
  • Immunization, Passive
  • Immunoglobulin G / blood
  • Immunoglobulin G / immunology
  • Lipopolysaccharides / chemistry
  • Lipopolysaccharides / immunology*
  • Neisseria meningitidis / immunology*
  • Pseudomonas Infections / prevention & control
  • Rabbits
  • Rats
  • Rats, Sprague-Dawley
  • Vaccines, Conjugate / immunology


  • Antibodies, Bacterial
  • Bacterial Outer Membrane Proteins
  • Bacterial Vaccines
  • Endotoxins
  • Escherichia coli Vaccines
  • Immune Sera
  • Immunoglobulin G
  • Lipopolysaccharides
  • Vaccines, Conjugate