Assays for insulin-like growth factors and their binding proteins: practicalities and pitfalls

J Pediatr. 1996 May;128(5 Pt 2):S52-7. doi: 10.1016/s0022-3476(96)70012-4.


Insulin-like growth factors (IGFs) are growth hormone-dependent anabolic peptides that circulate in biologic fluids complexed to a family of IGF binding proteins. Measurement of the serum concentrations of IGF peptides and IGF binding proteins has proved to be an effective means of evaluating functional growth hormone status and makes it possible to establish a diagnosis of IGF deficiency.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Blotting, Western
  • Enzyme-Linked Immunosorbent Assay
  • Growth Disorders / diagnosis*
  • Growth Disorders / drug therapy
  • Growth Hormone / deficiency
  • Growth Hormone-Releasing Hormone / therapeutic use
  • Humans
  • Insulin-Like Growth Factor Binding Proteins / blood*
  • Radioimmunoassay
  • Somatomedins / analysis*
  • Somatomedins / physiology


  • Insulin-Like Growth Factor Binding Proteins
  • Somatomedins
  • Growth Hormone
  • Growth Hormone-Releasing Hormone