Regulation of SNM1, an inducible Saccharomyces cerevisiae gene required for repair of DNA cross-links

Mol Gen Genet. 1996 Feb 5;250(2):162-8. doi: 10.1007/BF02174175.

Abstract

The interstrand cross-link repair gene SNM1 of Saccharomyces cerevisiae was examined for regulation in response to DNA-damaging agents. Induction of SNM1-lacZ fusions was detected in response to nitrogen mustard, cis-platinum (II) diamine dichloride, UV light, and 8-methoxypsoralen + UVA, but not after heat-shock treatment or incubation with 2-dimethylaminoethylchloride, methylmethane sulfonate or 4-nitroquinoline-N-oxide. The promoter of SNM1 contains a 15 bp motif, which shows homology to the DRE2 box of the RAD2 promoter. Similar motifs have been found in promoter regions of other damage-inducible DNA repair genes. Deletion of this motif results in loss of inducibility of SNM1. Also, a putative negative upstream regulation sequence was found to be responsible for repression of constitutive transcription of SNM1. Surprisingly, no inducibility of SNM1 was found after treatment with DNA-damaging agents in strains without an intact DUN1 gene, while regulation seems unchanged in sad1 mutants.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents / pharmacology
  • Base Sequence
  • Cisplatin / pharmacology
  • Consensus Sequence / genetics
  • Cross-Linking Reagents / chemistry
  • Cross-Linking Reagents / pharmacology
  • DNA Damage*
  • DNA Repair / genetics*
  • DNA, Fungal / drug effects
  • DNA, Fungal / radiation effects
  • DNA-Binding Proteins / genetics*
  • Endodeoxyribonucleases
  • Fungal Proteins / genetics*
  • Gene Expression Regulation, Fungal*
  • Methoxsalen / pharmacology
  • Molecular Sequence Data
  • Mutagenesis / genetics
  • Nuclear Proteins*
  • Promoter Regions, Genetic / genetics
  • Recombinant Fusion Proteins
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins*
  • Sequence Deletion
  • Sequence Homology, Nucleic Acid
  • TATA Box / genetics
  • Ultraviolet Rays
  • beta-Galactosidase / genetics
  • beta-Galactosidase / metabolism

Substances

  • Antineoplastic Agents
  • Cross-Linking Reagents
  • DNA, Fungal
  • DNA-Binding Proteins
  • Fungal Proteins
  • Nuclear Proteins
  • Recombinant Fusion Proteins
  • Saccharomyces cerevisiae Proteins
  • Endodeoxyribonucleases
  • PSO2 protein, S cerevisiae
  • beta-Galactosidase
  • Cisplatin
  • Methoxsalen