The str gene cluster for the biosynthesis of 5'-hydroxystreptomycin in Streptomyces glaucescens GLA.0 (ETH 22794): new operons and evidence for pathway-specific regulation by StrR

Mol Gen Genet. 1996 Apr 10;250(6):775-84. doi: 10.1007/BF02172990.


Two divergently oriented operons, strXU and strVW, located within the gene cluster for 5'-hydroxystreptomycin (5'-OH-Sm) biosynthesis in Streptomyces glaucescens strain GAL.0 (ETH 22794), were analysed by DNA sequencing and transcription/regulation studies. Three genes, strU and strVW, are conserved in a similar arrangement but in a different location within the str/sts gene cluster of the Sm-producing strain S. griseus N2-3-11. The four putative products resemble NDP-4-ketohexose 3,5-epimerases (StrX, M(r) 20.2 kDa), NAD(P)-dependent oxidoreductases (StrU, 45.6 kDa), and ABC-transporters (StrV, 61.8 kDa; StrW, 63.4 kDa). These genes are apparently involved in the biosynthesis of 5'-OH-Sm because the promoters of both operons are activated in trans by the activator StrR of S. griseus N2-3-11, when cloned in S. lividans 66 TK23. A sequence motif resembling the consensus sequence GTTCGActG(N)11CagTcGAAc for binding of StrR was identified within the intergenic region of strX and strV. Specific binding of StrR to this site was demonstrated by gel retardation assays using purified His*Tag-StrR.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP-Binding Cassette Transporters / chemistry
  • ATP-Binding Cassette Transporters / genetics
  • Amino Acid Sequence
  • Bacterial Proteins*
  • Base Sequence
  • Binding Sites
  • Cloning, Molecular
  • DNA Primers
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • Gene Expression Regulation, Bacterial / genetics
  • Genes, Bacterial
  • Membrane Proteins / chemistry
  • Membrane Proteins / genetics
  • Molecular Sequence Data
  • Multigene Family*
  • Operon / genetics*
  • Promoter Regions, Genetic / genetics
  • Regulon / genetics
  • Repetitive Sequences, Nucleic Acid
  • Reticulin / biosynthesis*
  • Streptomyces / genetics*
  • Streptomyces / metabolism
  • Transcription Factors / chemistry
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*
  • Transcription, Genetic / genetics


  • ATP-Binding Cassette Transporters
  • Bacterial Proteins
  • DNA Primers
  • DNA-Binding Proteins
  • Membrane Proteins
  • Reticulin
  • StrR protein, Streptomyces griseus
  • Transcription Factors

Associated data

  • GENBANK/AJ006985
  • GENBANK/X89010