Effects of cell cycle dependent histone H1 phosphorylation on chromatin structure and chromatin replication

Nucleic Acids Res. 1996 Apr 15;24(8):1420-7. doi: 10.1093/nar/24.8.1420.

Abstract

We have reconstituted salt-treated SV40 minichromosomes with differentially phosphorylated forms of histone H1 extracted from either G0-, S- or M-phase cells. Sedimentation studies revealed a clear difference between minichromosomes reconstituted with S-phase histone H1 compared with histone H1 from G0- or M-phase cells, indicating that the phosphorylation state of histone H1 has a direct effect on chromatin structure. Using reconstituted minichromosomes as substrate in the SV40 in vitro replication system, we measured a higher replication efficiency for SV40 minichromosomes reconstituted with S-phase histone H1 compared with G0- or M-phase histone H1. These data indicate that the chromatin structure induced by the phosphorylation of histone H1 influences the replication efficiency of SV40 minichromosomes in vitro.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Cycle
  • Cell Line
  • Chlorocebus aethiops
  • Chromatin / metabolism
  • Chromatin / ultrastructure*
  • DNA Replication*
  • DNA, Viral / metabolism
  • DNA, Viral / ultrastructure*
  • Histones / isolation & purification
  • Histones / metabolism*
  • Mitosis
  • Phosphorylation
  • Resting Phase, Cell Cycle
  • S Phase
  • Simian virus 40 / genetics*
  • Simian virus 40 / physiology

Substances

  • Chromatin
  • DNA, Viral
  • Histones