Afut1, a retrotransposon-like element from Aspergillus fumigatus

Nucleic Acids Res. 1996 Apr 15;24(8):1428-34. doi: 10.1093/nar/24.8.1428.

Abstract

A repeated DNA sequence used for epidemiological studies of the human opportunistic pathogen Aspergillus fumigatus has been characterized. It is a retroelement of 6914 bp in length, bounded by long terminal repeats of 282 bp, with sequence and features characteristic of retroviruses and retrotransposons. A 5 bp duplication site was found at its borders. This element, designated Afut1, encodes amino acid sequences homologous to the reverse transcriptase, RNase H and endonuclease encoded by the pol genes of retroelements. Comparison of the peptidic sequences with other putative polypeptides of fungal LTR retrotransposons showed that Afut1 is a member of the gypsy group. This is the first report of a transposable element in A.fumigatus. Afut1 is a defective element: the putative coding domains contain multiple stop codons due exclusively to transitions from C:G to T:A.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Aspergillus fumigatus / genetics*
  • Base Sequence
  • DNA, Fungal
  • Fungal Proteins / chemistry
  • Fungal Proteins / genetics*
  • Gene Dosage
  • Humans
  • Molecular Sequence Data
  • Repetitive Sequences, Nucleic Acid*
  • Retroelements*
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid

Substances

  • Afut1 protein, Aspergillus fumigatus
  • DNA, Fungal
  • Fungal Proteins
  • Retroelements

Associated data

  • GENBANK/L76085
  • GENBANK/L76086