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. 1996 Apr 15;24(8):1435-42.
doi: 10.1093/nar/24.8.1435.

Multiplex Messenger Assay: Simultaneous, Quantitative Measurement of Expression of Many Genes in the Context of T Cell Activation

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Free PMC article

Multiplex Messenger Assay: Simultaneous, Quantitative Measurement of Expression of Many Genes in the Context of T Cell Activation

K Bernard et al. Nucleic Acids Res. .
Free PMC article

Abstract

The hybridization signature approach, using colony filters and labeled complex probes, can provide high throughput measurement of gene activity. We describe here the implementation of this method to follow the expression levels of 47 genes in resting and activated T cells, as well as in epithelial cells. Using 4-fold spotting of colonies, imaging plate detection and various correction and normalization procedures, the technique is sensitive enough to quantify expression levels for sequences present at 0.005% abundance in the probe. Comparison with Northern blotting shows good consistency between the two methods. Upon activation of a T cell clone by an anti-CD3 antibody variations ranging from 2- to 20-fold are measured, some of which had not been reported previously. This 'multiplex messenger assay' method, performed using available commercial apparatus, can be used in many cases where simultaneous assessment of mRNA levels for many genes is of interest.

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References

    1. Anal Biochem. 1983 Jul 1;132(1):6-13 - PubMed
    1. Genet Anal. 1996 Jan;12(3-4):151-62 - PubMed
    1. J Immunol. 1986 Mar 15;136(6):1937-44 - PubMed
    1. Eur J Immunol. 1986 Dec;16(12):1533-8 - PubMed
    1. Proc Natl Acad Sci U S A. 1987 Mar;84(5):1374-8 - PubMed

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