Improved method for selecting RNA-binding activities in vivo

Nucleic Acids Res. 1996 Apr 15;24(8):1582-4. doi: 10.1093/nar/24.8.1582.


RNA challenge phages are modified versions of bacteriophage P22 that allow one to select directly for a specific RNA-protein interaction in vivo. The original construction method for generating a bacteriophage that encodes a specific RNA target requires two homologous recombination reactions between plasmids and phages in bacteria. An improved method is described that enables one to readily construct RNA challenge phages through a single homologous recombination reaction in vivo. We have applied the new method to construct a derivative of P22R17, an RNA challenge phage that undergoes lysogenic development in bacterial cells that express the bacteriophage R17/MS2 coat protein.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacteriophage P22 / genetics*
  • Base Sequence
  • Binding Sites
  • Molecular Sequence Data
  • Nucleic Acid Conformation
  • RNA, Viral / metabolism*
  • RNA-Binding Proteins / metabolism
  • Recombination, Genetic


  • RNA, Viral
  • RNA-Binding Proteins