Contrast-enhanced MR imaging assessment of tumor capillary permeability: effect of irradiation on delivery of chemotherapy

Radiology. 1996 Mar;198(3):893-8. doi: 10.1148/radiology.198.3.8628889.


Purpose: To assess the effect of x rays on tumor capillary permeability with macromolecular contrast medium (MMCM)-enhanced magnetic resonance (MR) imaging.

Materials and methods: One of paired R3230 mammary adenocarcinomas implanted in the flanks of 48 Fischer rats was treated with a single 5- or 15-Gy dose of x rays. After 1 or 3 days, MR imaging in 30 rats was performed unenhanced and enhanced with albumin-(gadolinium diethylenetriaminepentaacetic acid)30 (0.02 mmol gadolinium per kilogram body weight). Signal intensity enhancement was analyzed to estimate fractional blood volume and permeability-surface area product (PS). In 18 irradiated rats, 2.5 mg/kg cisplatin was injected intravenously, and fractional tumor platinum concentrations were measured spectrometrically.

Results: MR imaging showed highest capillary permeability 1 day after 15 Gy (PS 110% above control [P<.001]); PS was elevated 57% 1 day after 5 Gy and 63% 3 days after 15 Gy (P<.05). The largest mean tumor platinum fraction occurred 1 day after 15 Gy: 48% versus 20% (control)(P<.05).

Conclusion: Transient irradiation-induced increase in tumor capillary permeability to cisplatin can be quantified with MMCM-enhanced MR imaging.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Albumins*
  • Animals
  • Antineoplastic Agents / pharmacokinetics*
  • Antineoplastic Agents / therapeutic use
  • Capillary Permeability / radiation effects*
  • Cisplatin / pharmacokinetics*
  • Cisplatin / therapeutic use
  • Contrast Media*
  • Dose-Response Relationship, Radiation
  • Female
  • Gadolinium
  • Gadolinium DTPA*
  • Macromolecular Substances
  • Magnetic Resonance Imaging*
  • Mammary Neoplasms, Experimental / blood supply*
  • Mammary Neoplasms, Experimental / drug therapy
  • Mammary Neoplasms, Experimental / radiotherapy*
  • Organometallic Compounds*
  • Pentetic Acid / analogs & derivatives*
  • Rats
  • Rats, Inbred F344


  • Albumins
  • Antineoplastic Agents
  • Contrast Media
  • Macromolecular Substances
  • Organometallic Compounds
  • albumin-(gadolinium-DTPA)
  • Pentetic Acid
  • Gadolinium
  • Gadolinium DTPA
  • Cisplatin