Involvement of the oxidative pentose phosphate pathway in thiamine biosynthesis in Salmonella typhimurium

J Bacteriol. 1996 Mar;178(5):1476-9. doi: 10.1128/jb.178.5.1476-1479.1996.


purF mutants of Salmonella typhimurium are known to require a source of both purine and thiamine; however, exogenous pantothenate may be substituted for the thiamine requirement. We show here that the effect of pantothenate is prevented by blocks in the oxidative pentose phosphate pathway, gnd (encoding gluconate 6-phosphate [6-P] dehydrogenase) or zwf (encoding glucose 6-P dehydrogenase). We further show that the defects caused by these mutations can be overcome by increasing ribose 5-P, suggesting that ribose 5-P may play a role in the ability of pantothenate to substitute for thiamine.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aerobiosis
  • Anaerobiosis
  • Glucosephosphate Dehydrogenase / genetics
  • Glucosephosphate Dehydrogenase / metabolism
  • Models, Biological
  • Mutation
  • Oxidation-Reduction
  • Pantothenic Acid / metabolism
  • Pentose Phosphate Pathway*
  • Phosphogluconate Dehydrogenase / genetics
  • Phosphogluconate Dehydrogenase / metabolism
  • Pyrimidines / metabolism
  • Ribosemonophosphates / metabolism
  • Salmonella typhimurium / genetics
  • Salmonella typhimurium / metabolism*
  • Thiamine / biosynthesis*


  • Pyrimidines
  • Ribosemonophosphates
  • Pantothenic Acid
  • ribose-5-phosphate
  • Phosphogluconate Dehydrogenase
  • phosphogluconate dehydrogenase (decarboxylating)
  • Glucosephosphate Dehydrogenase
  • Thiamine