Abstract
purF mutants of Salmonella typhimurium are known to require a source of both purine and thiamine; however, exogenous pantothenate may be substituted for the thiamine requirement. We show here that the effect of pantothenate is prevented by blocks in the oxidative pentose phosphate pathway, gnd (encoding gluconate 6-phosphate [6-P] dehydrogenase) or zwf (encoding glucose 6-P dehydrogenase). We further show that the defects caused by these mutations can be overcome by increasing ribose 5-P, suggesting that ribose 5-P may play a role in the ability of pantothenate to substitute for thiamine.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Aerobiosis
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Anaerobiosis
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Glucosephosphate Dehydrogenase / genetics
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Glucosephosphate Dehydrogenase / metabolism
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Models, Biological
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Mutation
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Oxidation-Reduction
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Pantothenic Acid / metabolism
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Pentose Phosphate Pathway*
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Phosphogluconate Dehydrogenase / genetics
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Phosphogluconate Dehydrogenase / metabolism
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Pyrimidines / metabolism
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Ribosemonophosphates / metabolism
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Salmonella typhimurium / genetics
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Salmonella typhimurium / metabolism*
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Thiamine / biosynthesis*
Substances
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Pyrimidines
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Ribosemonophosphates
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Pantothenic Acid
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ribose-5-phosphate
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Phosphogluconate Dehydrogenase
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phosphogluconate dehydrogenase (decarboxylating)
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Glucosephosphate Dehydrogenase
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Thiamine