A yeast artificial chromosome-based map of the region of chromosome 20 containing the diabetes-susceptibility gene, MODY1, and a myeloid leukemia related gene

Proc Natl Acad Sci U S A. 1996 Apr 30;93(9):3937-41. doi: 10.1073/pnas.93.9.3937.


We have generated a physical map of human chromosome bands 20q11.2-20q13.1, a region containing a gene involved in the development of one form of early-onset, non-insulin-dependent diabetes mellitus, MODY1, as well as a putative myeloid tumor suppressor gene. The yeast artificial chromosome contig consists of 71 clones onto which 71 markers, including 20 genes, 5 expressed sequence tags, 32 simple tandem repeat DNA polymorphisms, and 14 sequence-tagged sites have been ordered. This region spans about 18 Mb, which represents about 40% of the physical length of 20q. Using this physical map, we have refined the location of MODY1 to a 13-centimorgan interval (approximately equal to 7 Mb) between D20S169 and D20S176. The myeloid tumor suppressor gene was localized to an 18-centimorgan interval (approximately equal to 13 Mb) between RPN2 and D20S17. This physical map will facilitate the isolation of MODY1 and the myeloid tumor suppressor gene.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Chromosome Mapping
  • Chromosomes, Artificial, Yeast*
  • Chromosomes, Human, Pair 20*
  • DNA / isolation & purification
  • DNA Primers
  • Diabetes Mellitus, Type 2 / genetics*
  • Disease Susceptibility
  • Electrophoresis, Agar Gel
  • Female
  • Genes, Tumor Suppressor*
  • Genetic Markers
  • Haplotypes
  • Humans
  • In Situ Hybridization, Fluorescence
  • Leukemia, Myeloid / genetics*
  • Male
  • Molecular Sequence Data
  • Pedigree
  • Polymorphism, Genetic
  • Repetitive Sequences, Nucleic Acid
  • Sequence Tagged Sites


  • DNA Primers
  • Genetic Markers
  • DNA

Associated data

  • GENBANK/G01520
  • GENBANK/G01521
  • GENBANK/G01522
  • GENBANK/G01523
  • GENBANK/G01524
  • GENBANK/G01525
  • GENBANK/G01526
  • GENBANK/G01527
  • GENBANK/G01528
  • GENBANK/G01529
  • GENBANK/G01530
  • GENBANK/G01531
  • GENBANK/G01532
  • GENBANK/G01533
  • GENBANK/G01534
  • GENBANK/G01535
  • GENBANK/G01536
  • GENBANK/G01537
  • GENBANK/G01538