Abstract
The adenovirus E4orf6 protein is shown here to interact with the cellular tumor suppressor protein p53 and to block p53-mediated transcriptional activation. The adenovirus protein inhibited the ability of p53 to bind to human TAFII31, a component of transcription factor IID (TFIID). Earlier work demonstrated that the interaction of p53 with TAFII31 involves a sequence near the NH2-terminus of p53, whereas the E4orf6-p53 interaction occurs within amino acids 318 to 360 of p53. Thus, the E4orf6 protein interacts at a site on p53 distinct from the domain that binds to TAFII31 but nevertheless inhibits the p53-TAFII31 interaction.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenoviridae / physiology
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Adenovirus E4 Proteins / immunology
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Adenovirus E4 Proteins / metabolism*
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Cell Line
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DNA / metabolism
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Genes, p53
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HeLa Cells
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Humans
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Immunoblotting
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Recombinant Fusion Proteins / metabolism
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TATA-Binding Protein Associated Factors*
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Trans-Activators / metabolism*
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Transcription Factor TFIID*
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Transcriptional Activation*
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Transfection
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Tumor Suppressor Protein p53 / chemistry
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Tumor Suppressor Protein p53 / metabolism*
Substances
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Adenovirus E4 Proteins
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Recombinant Fusion Proteins
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TAF9 protein, human
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TATA-Binding Protein Associated Factors
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Trans-Activators
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Transcription Factor TFIID
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Tumor Suppressor Protein p53
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DNA