Blockage by adenovirus E4orf6 of transcriptional activation by the p53 tumor suppressor

Science. 1996 Jun 7;272(5267):1470-3. doi: 10.1126/science.272.5267.1470.

Abstract

The adenovirus E4orf6 protein is shown here to interact with the cellular tumor suppressor protein p53 and to block p53-mediated transcriptional activation. The adenovirus protein inhibited the ability of p53 to bind to human TAFII31, a component of transcription factor IID (TFIID). Earlier work demonstrated that the interaction of p53 with TAFII31 involves a sequence near the NH2-terminus of p53, whereas the E4orf6-p53 interaction occurs within amino acids 318 to 360 of p53. Thus, the E4orf6 protein interacts at a site on p53 distinct from the domain that binds to TAFII31 but nevertheless inhibits the p53-TAFII31 interaction.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenoviridae / physiology
  • Adenovirus E4 Proteins / immunology
  • Adenovirus E4 Proteins / metabolism*
  • Cell Line
  • DNA / metabolism
  • Genes, p53
  • HeLa Cells
  • Humans
  • Immunoblotting
  • Recombinant Fusion Proteins / metabolism
  • TATA-Binding Protein Associated Factors*
  • Trans-Activators / metabolism*
  • Transcription Factor TFIID*
  • Transcriptional Activation*
  • Transfection
  • Tumor Suppressor Protein p53 / chemistry
  • Tumor Suppressor Protein p53 / metabolism*

Substances

  • Adenovirus E4 Proteins
  • Recombinant Fusion Proteins
  • TAF9 protein, human
  • TATA-Binding Protein Associated Factors
  • Trans-Activators
  • Transcription Factor TFIID
  • Tumor Suppressor Protein p53
  • DNA