A structural DNA binding protein of African swine fever virus with similarity to bacterial histone-like proteins

Arch Virol. 1996;141(2):301-13. doi: 10.1007/BF01718401.

Abstract

Here we describe an African swine fever virus (ASFV) protein encoded by the open reading frame 5-AR that shares structural and functional similarities with the family of bacterial histone-like proteins which include histone-like DNA binding proteins, integration host factor, and Bacillus phage SPO1 transcription factor, TF1. The ASFV 5-AR gene was cloned by PCR and expressed in E. coli. Monospecific antiserum prepared to the 5-AR bacterial expression product specifically immunoprecipitated a protein of approximately 11.6 kDa from ASFV infected swine macrophages at late times post infection. Additionally, the 5-AR expression product was strongly recognized by ASFV convalescent pig serum, indicating its antigenicity during natural infection. Cloned p11.6 bound both double and single stranded DNA-cellulose columns. Consistent with a DNA binding function, immunoelectronmicroscopy localized p11.6 to the virion nucleoid, To our knowledge, p11.6 is the first bacterial histone-like DNA-binding protein found in an animal virus or eukaryotic cell system.

MeSH terms

  • African Swine Fever Virus / genetics
  • African Swine Fever Virus / metabolism*
  • African Swine Fever Virus / ultrastructure
  • Amino Acid Sequence
  • Animals
  • Bacillus Phages / metabolism
  • Bacteria / metabolism*
  • Base Sequence
  • Cells, Cultured
  • Cloning, Molecular
  • DNA Primers
  • DNA-Binding Proteins / biosynthesis*
  • DNA-Binding Proteins / chemistry*
  • DNA-Binding Proteins / genetics
  • Escherichia coli
  • Histones / chemistry*
  • Macrophages / physiology
  • Macrophages / virology
  • Microscopy, Immunoelectron
  • Molecular Sequence Data
  • Molecular Weight
  • Open Reading Frames
  • Polymerase Chain Reaction
  • Protein Structure, Secondary
  • Sequence Homology, Amino Acid
  • Species Specificity
  • Swine
  • Virion / genetics
  • Virion / metabolism
  • Virion / ultrastructure

Substances

  • DNA Primers
  • DNA-Binding Proteins
  • Histones

Associated data

  • GENBANK/L18758
  • GENBANK/L18759
  • GENBANK/M86823
  • GENBANK/P08956
  • GENBANK/V00636
  • GENBANK/X11944
  • GENBANK/X67865
  • GENBANK/X71982