Complete sequence analysis of a gene (OS-9) ubiquitously expressed in human tissues and amplified in sarcomas

Mol Carcinog. 1996 Apr;15(4):270-5. doi: 10.1002/(SICI)1098-2744(199604)15:4<270::AID-MC4>3.0.CO;2-K.


Amplification and overexpression of genes involved in cellular growth control occur frequently in human tumors. Using a chromosome microdissection-based hybrid-selection strategy, we recently identified two novel genes (OS-9 and OS-4) within 12q13-15, a region frequently amplified in human cancers. We now report further characterization of the full-length OS-9 cDNA sequence consists of 2785 bp from which an open reading frame (ORF) with 667 amino-acid residues was deduced, The predicted polypeptide was water soluble and acidic. We also demonstrate that the OS-9 gene encoded a 2.8-kb mRNA transcribed in all 16 human tissues examined, suggesting that OS-9 is ubiquitously expressed in human tissues. OS-9 was co-amplified with CDK4 in three of five sarcoma tissues. Homology analysis of the amino-acid sequence reveals significant similarities between OS-9 and two ORFs deduced from genomic sequences in Caenorhabditis elegans and Saccharomyces cerevisiae. The region of similarity extended over 200 residues (approximately one-third of each ORF), and eight cysteines were conserved in all three ORFs. These observations suggest that this region comprises a functional domain present in a novel evolutionarily conserved gene family defined by OS-9.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Consensus Sequence
  • DNA, Neoplasm / genetics
  • Gene Amplification
  • Gene Expression Regulation, Neoplastic
  • Genes
  • Humans
  • Lectins
  • Molecular Sequence Data
  • Neoplasm Proteins / genetics*
  • RNA, Neoplasm / genetics*
  • Sarcoma / genetics*
  • Sequence Alignment
  • Sequence Homology, Amino Acid


  • DNA, Neoplasm
  • Lectins
  • Neoplasm Proteins
  • OS9 protein, human
  • RNA, Neoplasm

Associated data

  • GENBANK/U41635