Ability of paralyzed flagella mutants of Chlamydomonas to move

Cell Motil Cytoskeleton. 1996;33(2):88-94. doi: 10.1002/(SICI)1097-0169(1996)33:2<88::AID-CM2>3.0.CO;2-E.


Chlamydomonas mutants missing the central pair or radial spokes are paralyzed despite the fact that they have the full wild-type complement of functional dynein ATPases. We show here that these mutants can move under conditions of low ATP concentration, a combination of ATP and ADP, and a combination of ATP and ribose-modified ATP analogs. These conditions suggest an inhibitory role of ATP and that this inhibition can be relieved by ADP or analogs. The function of the central-pair/radial spoke complex may be to release this ATP inhibition in a controlled manner.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adenosine Diphosphate / analogs & derivatives
  • Adenosine Diphosphate / metabolism
  • Adenosine Diphosphate / pharmacology
  • Adenosine Triphosphate / analogs & derivatives
  • Adenosine Triphosphate / metabolism
  • Adenosine Triphosphate / pharmacology
  • Animals
  • Cell Movement / physiology*
  • Chlamydomonas / cytology*
  • Chlamydomonas / genetics
  • Chlamydomonas / ultrastructure
  • Dyneins / genetics
  • Dyneins / metabolism
  • Flagella / chemistry
  • Flagella / physiology*
  • Microscopy, Video
  • Mutation / physiology


  • Adenosine Diphosphate
  • Adenosine Triphosphate
  • Dyneins