Cloning and characterization of the Saccharomyces cerevisiae C-22 sterol desaturase gene, encoding a second cytochrome P-450 involved in ergosterol biosynthesis

Gene. 1996 Feb 22;169(1):105-9. doi: 10.1016/0378-1119(95)00770-9.

Abstract

The ERG5 gene from Saccharomyces cerevisiae was cloned by complementation of an erg5-1 mutation using a negative selection protocol involving screening for nystatin-sensitive transformants. ERG5 is the putative gene encoding the C-22 sterol desaturase required in ergosterol biosynthesis. The functional gene was localized to a 2.15-kb SacI-EcoRI DNA fragment containing an open reading frame of 538 amino acids (aa). ERG5 contains a 10-aa motif consistent with its role as a cytochrome P-450 (CyP450) enzyme and is similar to a number of mammalian CyP450 enzymes. Gene disruption demonstrates that ERG5 is not essential for cell viability.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Chromosome Mapping
  • Cloning, Molecular
  • Cytochrome P-450 Enzyme System / genetics*
  • Ergosterol / biosynthesis*
  • Gene Expression
  • Genes, Fungal*
  • Molecular Sequence Data
  • Oxidoreductases / genetics*
  • Restriction Mapping
  • Saccharomyces cerevisiae / enzymology*
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae Proteins
  • Sequence Homology, Amino Acid

Substances

  • Saccharomyces cerevisiae Proteins
  • Cytochrome P-450 Enzyme System
  • Oxidoreductases
  • ERG5 protein, S cerevisiae
  • Ergosterol

Associated data

  • GENBANK/U34636