Sequence of the Bacillus stearothermophilus gene encoding aspartokinase II

R Cantoni; M Labó; E De Rossi; G Riccardi

doi:10.1016/0378-1119(95)00834-9

Sequence of the Bacillus stearothermophilus gene encoding aspartokinase II

Gene. 1996 Feb 22;169(1):135-6. doi: 10.1016/0378-1119(95)00834-9.

Authors

R Cantoni¹, M Labó, E De Rossi, G Riccardi

Affiliation

¹ Dipartimento di Genetica e Microbiologia, University of Pavia, Italy.

PMID: 8635739
DOI: 10.1016/0378-1119(95)00834-9

Abstract

The complete nucleotide sequence of the gene encoding aspartokinase (Ask) II from thermophilic Bacillus stearothermophilus has been determined. Degenerate oligodeoxyribonucleotides primed the amplification of a 932-bp gene. This sequence was successively used for constructing new primers applied in inverse polymerase chain reaction using, as template, self-ligating DNA fragments. The deduced amino-acid sequence is 68.7% identical with the sequence of the Bacillus sp. strain MGA3 Ask II.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Aspartate Kinase / genetics*
Base Sequence
DNA Primers / chemistry
DNA, Bacterial / genetics
Genes, Bacterial
Geobacillus stearothermophilus / enzymology
Geobacillus stearothermophilus / genetics*
Molecular Sequence Data

Substances

DNA Primers
DNA, Bacterial
Aspartate Kinase

Associated data

GENBANK/L46351