Microbial genes homologous to the peptidyl-tRNA hydrolase-encoding gene of Escherichia coli

Gene. 1996 Feb 22;169(1):97-100. doi: 10.1016/0378-1119(95)00823-3.


We have cloned and determined the nucleotide (nt) sequences of the genes encoding peptidyl-tRNA hydrolase (Pth) homologues of Salmonella typhi (St) and the Lyme disease spirochaete, Borrelia burgdorferi (Bb). We also completed the nt sequence of a pth homologous gene contained in a Chlamydia trachomatis (Ct) clone identified in the databanks. The open reading frames (ORFs) of the Pth homologues encode putative polypeptides of 194 (St), 188 (Bb) and 194 (Ct) amino acids exhibiting significant identity with Escherichia coli (Ec) Pth. Together with the products of two previously unidentified ORFs from Bacillus subtilis and Saccharomyces cerevisiae, and the recently recognized Haemophilus influenzae and Mycoplasma genitalium pth genes, these seven putative polypeptides and the Ec Pth form a group of homologous basic proteins spanning eubacteria and eukaryota which can be defined by at least three conserved regions. Previously known Ec pth mutations were located in highly conserved residues.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Borrelia burgdorferi Group / enzymology*
  • Borrelia burgdorferi Group / genetics
  • Carboxylic Ester Hydrolases / genetics*
  • Chlamydia trachomatis / enzymology*
  • Chlamydia trachomatis / genetics
  • Cloning, Molecular
  • Escherichia coli / genetics
  • Genes, Bacterial*
  • Molecular Sequence Data
  • Phylogeny
  • Salmonella typhi / enzymology*
  • Salmonella typhi / genetics
  • Sequence Alignment
  • Sequence Homology, Amino Acid


  • Carboxylic Ester Hydrolases
  • aminoacyl-tRNA hydrolase

Associated data

  • GENBANK/L32144
  • GENBANK/U31570
  • GENBANK/U31571