Characterization of an Arabidopsis thaliana gene that defines a new class of putative plant receptor kinases with an extracellular lectin-like domain

J Mol Biol. 1996 May 24;258(5):778-88. doi: 10.1006/jmbi.1996.0286.

Abstract

We have characterized an Arabidopsis receptor-like serine/threonine kinase gene, Ath.lecRK1 (Arabidopsis thaliana lectin-receptor kinase), defining a new and putatively important class of plant receptor kinases. Structural features of the predicted polypeptide include an amino-terminal membrane-targeting signal sequence, a legume lectin-like extracellular domain, a single membrane-spanning domain, and a characteristic serine/threonine protein kinase domain. A recombinant protein containing the kinase domain can be autophosphorylated on a serine residue. Ath.lecRK1 is a member of a gene family of at least two closely related genes. Northern blot analysis indicates that the Ath.lecRK1 gene is weakly expressed in a variety of organs and is regulated in Arabidopsis cell suspension cultures according to the growth phase of cells. The role this new class of plant receptor kinase could play is discussed with regard to the transduction of oligosaccharide and plant hormone signals.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / genetics*
  • Arabidopsis Proteins*
  • Base Sequence
  • Chemical Phenomena
  • Chemistry, Physical
  • Cloning, Molecular
  • Gene Expression Regulation
  • Genes, Plant*
  • Lectins / chemistry*
  • Molecular Sequence Data
  • Phosphorylation
  • Plant Lectins
  • Plant Proteins / chemistry
  • Plant Proteins / genetics*
  • Polymerase Chain Reaction
  • Protein Kinases / chemistry
  • Protein Processing, Post-Translational
  • Protein Structure, Tertiary
  • Protein-Serine-Threonine Kinases / chemistry
  • Protein-Serine-Threonine Kinases / genetics*
  • Sequence Alignment
  • Sequence Homology, Amino Acid

Substances

  • Arabidopsis Proteins
  • Lectins
  • Plant Lectins
  • Plant Proteins
  • Protein Kinases
  • ATHLECRK protein, Arabidopsis
  • Protein-Serine-Threonine Kinases

Associated data

  • GENBANK/AF001168
  • GENBANK/X91630