Enhancing Effects of Epidermal Growth Factor on Human Squamous Cell Carcinoma Motility and Matrix Degradation but Not Growth

Tumour Biol. 1996;17(3):168-75. doi: 10.1159/000217979.

Abstract

In order to ascertain the effects of epidermal growth factor (EGF) on human cancer invasion abilities, three cell lines of human oral squamous cell carcinoma were studied using a phagokinetic track assay and zymography. EGF (1-100 ng/ml) was found to inhibit the growth but enhance the random motility of all three cell lines in a concentration-dependent fashion. Exposure to EGF, dose-dependently, led to an increased production of urokinase-type plasminogen activator and M(r) 92 kD matrix metalloproteinase by the same cells. These results strongly suggest that EGF may promote human squamous cell carcinoma invasion and metastasis.

MeSH terms

  • Carcinoma, Squamous Cell / pathology*
  • Cell Division
  • Cell Movement / drug effects
  • Epidermal Growth Factor / pharmacology*
  • Extracellular Matrix / metabolism
  • Humans
  • Metalloendopeptidases / metabolism
  • Tumor Cells, Cultured
  • Urokinase-Type Plasminogen Activator / metabolism

Substances

  • Epidermal Growth Factor
  • Urokinase-Type Plasminogen Activator
  • Metalloendopeptidases