Expression of RHD and RHCE gene products using retroviral transduction of K562 cells establishes the molecular basis of Rh blood group antigens

Blood. 1996 Apr 1;87(7):2968-73.


Retroviral-mediated gene transfer using cDNA transcripts of the RHD and RHCE genes resulted in the isolation of K562 clones expressing D and G or c and E antigens, respectively. These results represent the first direct demonstration that the RHD gene encodes the D and G antigens and the RHCE gene encodes the c and E antigens. Both c and E antigens were expressed after transduction of K562 cells with a single cDNA, indicating that the c antigen does not arise by alternative splicing (exon skipping) of the product of the RHCE gene, as has been suggested.

MeSH terms

  • Antigens / genetics*
  • Antigens / immunology
  • Base Sequence
  • Blood Group Antigens / genetics*
  • Blood Group Antigens / immunology
  • Cloning, Molecular
  • Flow Cytometry
  • Gene Transfer Techniques
  • Humans
  • Molecular Sequence Data
  • Tumor Cells, Cultured


  • Antigens
  • Blood Group Antigens