Identification and Mapping of Human cDNAs Homologous to Drosophila Mutant Genes Through EST Database Searching

Nat Genet. 1996 Jun;13(2):167-74. doi: 10.1038/ng0696-167.

Abstract

Cross-species comparison is an effective tool used to identify genes and study their function in both normal and pathological conditions. We have applied the power of Drosophila genetics to the vast resource of human cDNAs represented in the expressed sequence tag (EST) database (dbEST) to identify novel human genes of high biological interest. Sixty-six human cDNAs showing significant homology to genes causing Drosophila mutant phenotypes were identified by screening dbEST using the "text string' option, and their map position was determined using both fluorescence in situ hybridization (FISH) and radiation hybrid mapping. Comparison between these genes and their putative partners in Drosophila may provide important insights into their function in mammals. Furthermore, integration of these genes into the transcription map of the human genome contributes to the positional candidate approach for disease gene identification.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Chromosome Mapping / methods*
  • Computer Communication Networks
  • DNA, Complementary
  • Databases, Factual*
  • Drosophila melanogaster / genetics*
  • Genes, Insect
  • Humans
  • Hybrid Cells / radiation effects
  • In Situ Hybridization, Fluorescence
  • Models, Genetic
  • Molecular Sequence Data
  • Mutation
  • Phenotype
  • Sequence Alignment / methods
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid*

Substances

  • DNA, Complementary

Associated data

  • GENBANK/G30939
  • GENBANK/G30940
  • GENBANK/G31158
  • GENBANK/G31159
  • GENBANK/G31160
  • GENBANK/G31161
  • GENBANK/G31203
  • GENBANK/G31204