Interleukin-8 inhibits non-small cell lung cancer proliferation: a possible role for regulation of tumor growth by autocrine and paracrine pathways

J Interferon Cytokine Res. 1996 Jan;16(1):53-60. doi: 10.1089/jir.1996.16.53.


Interleukin-8 (IL-8) is an 8 kD chemokine and angiogenic factor produced by alveolar macrophages, endothelial cells, monocytes, fibroblasts, T lymphocytes, and epithelial cells in response to a variety of stimuli, including LPS, TNF-alpha, IL-1, IL-7, and hypoxia. Pulmonary tumors produce a variety of growth factors and cytokines that may act in both autocrine and paracrine fashion. A549, a well-characterized human lung adenocarcinoma line, was cloned for different levels of IL-8 production by limiting dilution. Clone 3B4 produced 361 +/- 73 pg/ml, and clone 2B2 produced 7818 +/- 614 pg/ml of IL-8 (p = 0.003). Clone 3B4 proliferated at 1.7 times the rate of 2B2. Anti-IL-8 reversed the decrement in proliferation of clone 2B2 by 50%, but recombinant IL-8 decreased the proliferation of 3B4 by 40-55% compared with control. In addition to A549, three other non-small cell lung cancer (NSCLC) lines showed significantly decreased proliferation in response to exogenous recombinant IL-8 (5-30 ng/ml; p < 0.05). These findings suggest that in addition to its chemotactic and angiogenic activities, IL-8 may inhibit lung tumor proliferation by both autocrine and paracrine pathways.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antigens, CD / analysis
  • Antineoplastic Agents / therapeutic use*
  • Base Sequence
  • Carcinoma, Non-Small-Cell Lung / drug therapy*
  • Cell Division / drug effects
  • Clone Cells
  • Hormones / physiology*
  • Humans
  • Interleukin-8 / genetics
  • Interleukin-8 / therapeutic use*
  • Lung Neoplasms / drug therapy*
  • Molecular Sequence Data
  • RNA, Messenger / biosynthesis
  • Receptors, Interleukin / analysis
  • Receptors, Interleukin-8A
  • Recombinant Proteins / therapeutic use
  • Tumor Cells, Cultured


  • Antigens, CD
  • Antineoplastic Agents
  • Hormones
  • Interleukin-8
  • RNA, Messenger
  • Receptors, Interleukin
  • Receptors, Interleukin-8A
  • Recombinant Proteins