Suppression of superoxide anion production by interleukin-10 is accompanied by a downregulation of the genes for subunit proteins of NADPH oxidase

Exp Hematol. 1996 Feb;24(2):151-7.


Interleukin-10 (IL-10) inhibited the production of superoxide anion (02-) by both unactivated and interferon-gamma (IFN-gamma)-activated human monocytes. Simultaneous addition of IL-10 with IFN-gamma at the start of incubation was necessary for an optimal inhibitory effect. The degree of inhibition was substantially comparable to that of IL-4, and the combination of suboptimal concentrations of IL-10 and IL-4 produced an additive effect. A similar effect was also obtained when viral IL-10 (vIL-10) was used instead of IL-10. The inhibitory effect of IL-10 was accompanied by the reduced accumulation of transcripts for heavy chain subunit of cytochrome b558 (gp9l-phox) and 47-kD cytosolic factor (p47-phox), components of the O2--generating NADPH oxidase system. Reduction of the mRNAs was distinct within 24 hours. On the other hand, the induced O2- production by human monocytic leukemia cell lines (THP-1 and HL60) was not inhibited by IL-10. The amount of gp9l-phox and p47-phox mRNAs remained unchanged even in the presence of excess amount of IL-1O. Taken together, these results suggest that IL-10 inhibits 02- production by downregulation of the gp9l-phox and p47-phox genes in human monocytes.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Depression, Chemical
  • Enzyme Induction / drug effects
  • Gene Expression Regulation, Leukemic / drug effects
  • HL-60 Cells / drug effects
  • HL-60 Cells / metabolism
  • Humans
  • Interleukin-10 / pharmacology*
  • Interleukin-4 / pharmacology
  • Leukemia, Myelomonocytic, Acute / genetics
  • Leukemia, Myelomonocytic, Acute / pathology
  • Leukemia, Promyelocytic, Acute / genetics
  • Leukemia, Promyelocytic, Acute / pathology
  • Membrane Glycoproteins / biosynthesis*
  • Membrane Glycoproteins / genetics
  • Membrane Transport Proteins*
  • Monocytes / drug effects*
  • Monocytes / metabolism
  • NADH, NADPH Oxidoreductases / biosynthesis*
  • NADH, NADPH Oxidoreductases / genetics
  • NADPH Dehydrogenase / biosynthesis
  • NADPH Dehydrogenase / genetics
  • NADPH Oxidase 2
  • NADPH Oxidases
  • Neoplasm Proteins / biosynthesis
  • Neoplasm Proteins / genetics
  • Phosphoproteins / biosynthesis*
  • Phosphoproteins / genetics
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • Recombinant Proteins / pharmacology
  • Superoxides / metabolism*


  • Membrane Glycoproteins
  • Membrane Transport Proteins
  • Neoplasm Proteins
  • Phosphoproteins
  • RNA, Messenger
  • Recombinant Proteins
  • neutrophil cytosol factor 67K
  • Superoxides
  • Interleukin-10
  • Interleukin-4
  • NADH, NADPH Oxidoreductases
  • CYBB protein, human
  • NADPH Oxidase 2
  • NADPH Oxidases
  • CYBA protein, human
  • neutrophil cytosolic factor 1
  • NADPH Dehydrogenase